CBSX3-Trxo-2 regulates ROS generation of mitochondrial complex II (succinate dehydrogenase) in Arabidopsis.
Identifieur interne : 000051 ( Main/Exploration ); précédent : 000050; suivant : 000052CBSX3-Trxo-2 regulates ROS generation of mitochondrial complex II (succinate dehydrogenase) in Arabidopsis.
Auteurs : Jin Seok Shin [Corée du Sud] ; Won Mi So [Corée du Sud] ; Soo Youn Kim [Corée du Sud] ; Minsoo Noh [Corée du Sud] ; Sujin Hyoung [Corée du Sud] ; Kyoung Shin Yoo [États-Unis] ; Jeong Sheop Shin [Corée du Sud]Source :
- Plant science : an international journal of experimental plant biology [ 1873-2259 ] ; 2020.
Abstract
Despite being toxic at a high concentrations, reactive oxygen species (ROS) play a pivotal role as signaling molecules in responses to stress and regulation of plant development. The mitochondrial electron transport chain (ETC) is the major source of ROS in cells. Although the regulation of ROS in mitochondria has been well elucidated, the protein-protein interaction-based regulation of ETC members has not been well elucidated. In this study, we identified a CBS domain-containing protein, CBSX3, and found that CBSX3 activates o-type thioredoxin (Trx-o2) in mitochondria. In addition, we found that Trx-o2 interacts with SDH1, a subunit of ETC complex II. Knockdown (KD) of CBSX3 revealed anther indehiscence due to deficient lignin deposition caused by insufficient ROS accumulation, and increased expression of genes related to cell cycle and accelerated plant growth. However, in the CBSX3-overexpression plants, ROS accumulation increased, and cell cycle-related gene expression decreased, and thereby plant growth was retarded and leaf size decreased. Moreover, KD of CBSX3 and Trx-o2 conferred resistance to mitochondria ETC inhibitors in terms of ROS release. Taken together, we suggest that CBSX3-Trx-o2 is a ROS generation regulator of mitochondria in plants and plays an important role in regulating plant development and the redox system.
DOI: 10.1016/j.plantsci.2020.110458
PubMed: 32234226
Affiliations:
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Electronic address: jsshin@korea.ac.kr.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Life Sciences, Korea University, Seoul 02841</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author></analytic><series><title level="j">Plant science : an international journal of experimental plant biology</title><idno type="eISSN">1873-2259</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Despite being toxic at a high concentrations, reactive oxygen species (ROS) play a pivotal role as signaling molecules in responses to stress and regulation of plant development. The mitochondrial electron transport chain (ETC) is the major source of ROS in cells. Although the regulation of ROS in mitochondria has been well elucidated, the protein-protein interaction-based regulation of ETC members has not been well elucidated. In this study, we identified a CBS domain-containing protein, CBSX3, and found that CBSX3 activates o-type thioredoxin (Trx-o2) in mitochondria. In addition, we found that Trx-o2 interacts with SDH1, a subunit of ETC complex II. Knockdown (KD) of CBSX3 revealed anther indehiscence due to deficient lignin deposition caused by insufficient ROS accumulation, and increased expression of genes related to cell cycle and accelerated plant growth. However, in the CBSX3-overexpression plants, ROS accumulation increased, and cell cycle-related gene expression decreased, and thereby plant growth was retarded and leaf size decreased. Moreover, KD of CBSX3 and Trx-o2 conferred resistance to mitochondria ETC inhibitors in terms of ROS release. Taken together, we suggest that CBSX3-Trx-o2 is a ROS generation regulator of mitochondria in plants and plays an important role in regulating plant development and the redox system.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32234226</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-2259</ISSN><JournalIssue CitedMedium="Internet"><Volume>294</Volume><PubDate><Year>2020</Year><Month>May</Month></PubDate></JournalIssue><Title>Plant science : an international journal of experimental plant biology</Title><ISOAbbreviation>Plant Sci</ISOAbbreviation></Journal><ArticleTitle>CBSX3-Trxo-2 regulates ROS generation of mitochondrial complex II (succinate dehydrogenase) in Arabidopsis.</ArticleTitle><Pagination><MedlinePgn>110458</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0168-9452(20)30060-1</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.plantsci.2020.110458</ELocationID><Abstract><AbstractText>Despite being toxic at a high concentrations, reactive oxygen species (ROS) play a pivotal role as signaling molecules in responses to stress and regulation of plant development. The mitochondrial electron transport chain (ETC) is the major source of ROS in cells. Although the regulation of ROS in mitochondria has been well elucidated, the protein-protein interaction-based regulation of ETC members has not been well elucidated. In this study, we identified a CBS domain-containing protein, CBSX3, and found that CBSX3 activates o-type thioredoxin (Trx-o2) in mitochondria. In addition, we found that Trx-o2 interacts with SDH1, a subunit of ETC complex II. Knockdown (KD) of CBSX3 revealed anther indehiscence due to deficient lignin deposition caused by insufficient ROS accumulation, and increased expression of genes related to cell cycle and accelerated plant growth. However, in the CBSX3-overexpression plants, ROS accumulation increased, and cell cycle-related gene expression decreased, and thereby plant growth was retarded and leaf size decreased. Moreover, KD of CBSX3 and Trx-o2 conferred resistance to mitochondria ETC inhibitors in terms of ROS release. Taken together, we suggest that CBSX3-Trx-o2 is a ROS generation regulator of mitochondria in plants and plays an important role in regulating plant development and the redox system.</AbstractText><CopyrightInformation>Copyright © 2020 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Shin</LastName><ForeName>Jin Seok</ForeName><Initials>JS</Initials><AffiliationInfo><Affiliation>Division of Life Sciences, Korea University, Seoul 02841, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>So</LastName><ForeName>Won Mi</ForeName><Initials>WM</Initials><AffiliationInfo><Affiliation>Division of Life Sciences, Korea University, Seoul 02841, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Soo Youn</ForeName><Initials>SY</Initials><AffiliationInfo><Affiliation>Division of Life Sciences, Korea University, Seoul 02841, Republic of Korea; Bionics Inc., Seongdong-gu, Seoul 04778, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Noh</LastName><ForeName>Minsoo</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Division of Life Sciences, Korea University, Seoul 02841, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hyoung</LastName><ForeName>Sujin</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Division of Life Sciences, Korea University, Seoul 02841, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yoo</LastName><ForeName>Kyoung Shin</ForeName><Initials>KS</Initials><AffiliationInfo><Affiliation>Division of Life Sciences, Korea University, Seoul 02841, Republic of Korea; Department of Neuroscience, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shin</LastName><ForeName>Jeong Sheop</ForeName><Initials>JS</Initials><AffiliationInfo><Affiliation>Division of Life Sciences, Korea University, Seoul 02841, Republic of Korea. Electronic address: jsshin@korea.ac.kr.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>02</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>Ireland</Country><MedlineTA>Plant Sci</MedlineTA><NlmUniqueID>9882015</NlmUniqueID><ISSNLinking>0168-9452</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">CBS domain</Keyword><Keyword MajorTopicYN="N">Lignification</Keyword><Keyword MajorTopicYN="N">Mitochondria</Keyword><Keyword MajorTopicYN="N">ROS</Keyword><Keyword MajorTopicYN="N">Succinate dehydrogenase</Keyword><Keyword MajorTopicYN="N">Thioredoxin</Keyword></KeywordList><CoiStatement>Declaration of Competing Interest The authors declare that they have no conflict of interest.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>11</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>01</Month><Day>31</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>02</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>4</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>4</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>4</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32234226</ArticleId><ArticleId IdType="pii">S0168-9452(20)30060-1</ArticleId><ArticleId IdType="doi">10.1016/j.plantsci.2020.110458</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Corée du Sud</li><li>États-Unis</li></country><region><li>Région capitale de Séoul</li><li>Texas</li></region><settlement><li>Séoul</li></settlement></list><tree><country name="Corée du Sud"><region name="Région capitale de Séoul"><name sortKey="Shin, Jin Seok" sort="Shin, Jin Seok" uniqKey="Shin J" first="Jin Seok" last="Shin">Jin Seok Shin</name></region><name sortKey="Hyoung, Sujin" sort="Hyoung, Sujin" uniqKey="Hyoung S" first="Sujin" last="Hyoung">Sujin Hyoung</name><name sortKey="Kim, Soo Youn" sort="Kim, Soo Youn" uniqKey="Kim S" first="Soo Youn" last="Kim">Soo Youn Kim</name><name sortKey="Noh, Minsoo" sort="Noh, Minsoo" uniqKey="Noh M" first="Minsoo" last="Noh">Minsoo Noh</name><name sortKey="Shin, Jeong Sheop" sort="Shin, Jeong Sheop" uniqKey="Shin J" first="Jeong Sheop" last="Shin">Jeong Sheop Shin</name><name sortKey="So, Won Mi" sort="So, Won Mi" uniqKey="So W" first="Won Mi" last="So">Won Mi So</name></country><country name="États-Unis"><region name="Texas"><name sortKey="Yoo, Kyoung Shin" sort="Yoo, Kyoung Shin" uniqKey="Yoo K" first="Kyoung Shin" last="Yoo">Kyoung Shin Yoo</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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